Journal: PLoS ONE
Article Title: Yohimbine Enhances Protection of Berberine against LPS-Induced Mouse Lethality through Multiple Mechanisms
doi: 10.1371/journal.pone.0052863
Figure Lengend Snippet: Phosphorylation of IRF3, Tyk2 and STAT1 were assessed by Western blot. The expression of IFN-β and IP-10 mRNA were detected by real-time RT-PCR. n = 6−8 . *P <0.05 compared with control group; # P <0.05 compared with LPS group. $ P <0.05 compared with Ber+LPS group.
Article Snippet: Antibodies, including IκBα, ERK, JNK, p38MAPK, NF-κB, IRF3, Tyk2, STAT1, phosphorylated IκBα (p-IκBα), phosphorylated JNK (p-JNK), phosphorylated ERK(p-ERK), phosphorylated p38MAPK (p-p38 MAPK), phosphorylated IRF3(p-IRF3), phosphorylated Tyk2 (p-Tyk2), phosphorylated STAT1(p-STAT1) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and horseradish peroxidase -labeled secondary antibody were from Cell Signaling Technology, Inc. (Danvers, MA, USA).
Techniques: Phospho-proteomics, Western Blot, Expressing, Quantitative RT-PCR, Control